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Evaluation of the in vitro antitumor efficacy of Allo CAR70-NKT cells (A) Experimental design to study the in vitro antitumor efficacy of Allo CAR70-NKT cells against human RCC cell lines. (B) Schematics showing the indicated human tumor cell lines. (C) Tumor cell killing data at 24 h (n = 4). (D) ELISA analyses of IFN-γ and TNF-α production by Allo CAR70-NKT cells after 24-hour coculture with <t>786-O-FG</t> cells (n = 4). (E) Experimental design to Study the tumor cell killing mechanisms of Allo CAR70-NKT cells mediated by NKRs. (F) Tumor cell killing data at 24 h (E:T ratio = 0.5:1; n = 4). Representative of 3 experiments. Data are presented as the mean ± SEM. ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 by Student’s t test (D) or one-way ANOVA (C and F).
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Evaluation of the in vitro antitumor efficacy of Allo CAR70-NKT cells (A) Experimental design to study the in vitro antitumor efficacy of Allo CAR70-NKT cells against human RCC cell lines. (B) Schematics showing the indicated human tumor cell lines. (C) Tumor cell killing data at 24 h (n = 4). (D) ELISA analyses of IFN-γ and TNF-α production by Allo CAR70-NKT cells after 24-hour coculture with <t>786-O-FG</t> cells (n = 4). (E) Experimental design to Study the tumor cell killing mechanisms of Allo CAR70-NKT cells mediated by NKRs. (F) Tumor cell killing data at 24 h (E:T ratio = 0.5:1; n = 4). Representative of 3 experiments. Data are presented as the mean ± SEM. ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 by Student’s t test (D) or one-way ANOVA (C and F).
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Evaluation of the in vitro antitumor efficacy of Allo CAR70-NKT cells (A) Experimental design to study the in vitro antitumor efficacy of Allo CAR70-NKT cells against human RCC cell lines. (B) Schematics showing the indicated human tumor cell lines. (C) Tumor cell killing data at 24 h (n = 4). (D) ELISA analyses of IFN-γ and TNF-α production by Allo CAR70-NKT cells after 24-hour coculture with <t>786-O-FG</t> cells (n = 4). (E) Experimental design to Study the tumor cell killing mechanisms of Allo CAR70-NKT cells mediated by NKRs. (F) Tumor cell killing data at 24 h (E:T ratio = 0.5:1; n = 4). Representative of 3 experiments. Data are presented as the mean ± SEM. ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 by Student’s t test (D) or one-way ANOVA (C and F).
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Evaluation of the in vitro antitumor efficacy of Allo CAR70-NKT cells (A) Experimental design to study the in vitro antitumor efficacy of Allo CAR70-NKT cells against human RCC cell lines. (B) Schematics showing the indicated human tumor cell lines. (C) Tumor cell killing data at 24 h (n = 4). (D) ELISA analyses of IFN-γ and TNF-α production by Allo CAR70-NKT cells after 24-hour coculture with 786-O-FG cells (n = 4). (E) Experimental design to Study the tumor cell killing mechanisms of Allo CAR70-NKT cells mediated by NKRs. (F) Tumor cell killing data at 24 h (E:T ratio = 0.5:1; n = 4). Representative of 3 experiments. Data are presented as the mean ± SEM. ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 by Student’s t test (D) or one-way ANOVA (C and F).

Journal: STAR Protocols

Article Title: Protocol to generate human stem cell-derived CD70-directed allogeneic CAR-NKT cells for treating renal cell carcinoma

doi: 10.1016/j.xpro.2025.104340

Figure Lengend Snippet: Evaluation of the in vitro antitumor efficacy of Allo CAR70-NKT cells (A) Experimental design to study the in vitro antitumor efficacy of Allo CAR70-NKT cells against human RCC cell lines. (B) Schematics showing the indicated human tumor cell lines. (C) Tumor cell killing data at 24 h (n = 4). (D) ELISA analyses of IFN-γ and TNF-α production by Allo CAR70-NKT cells after 24-hour coculture with 786-O-FG cells (n = 4). (E) Experimental design to Study the tumor cell killing mechanisms of Allo CAR70-NKT cells mediated by NKRs. (F) Tumor cell killing data at 24 h (E:T ratio = 0.5:1; n = 4). Representative of 3 experiments. Data are presented as the mean ± SEM. ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 by Student’s t test (D) or one-way ANOVA (C and F).

Article Snippet: Human renal cell carcinoma cell line 786-O , ATCC , CRL-1932.

Techniques: In Vitro, Enzyme-linked Immunosorbent Assay